positive control human liver tissue sections hf-314 (Zyagen Inc)
90
Structured Review
Zyagen Inc
positive control human liver tissue sections hf-314
Positive Control Human Liver Tissue Sections Hf 314, supplied by Zyagen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+liver+tissue+sections/positive+control+human+liver+tissue+sections+hf+314/us11608510-1471-10-18
Average 90 stars, based on 1 article reviews
Positive Control Human Liver Tissue Sections Hf 314, supplied by Zyagen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+liver+tissue+sections/positive+control+human+liver+tissue+sections+hf+314/us11608510-1471-10-18
Average 90 stars, based on 1 article reviews
positive control human liver tissue sections hf-314 - by Bioz Stars,
2026-09
90/100 stars
Images
Related Articles
Staining:Article Title: Bioengineered AAV Capsids with Combined High Human Liver Transduction In Vivo and Unique Humoral Seroreactivity Article Snippet: Modifications included fixing slides in ice-cold methanol for 10 min rather than acetone at room temperature; blocking with 10% rather than 5% donkey serum (Cat. no. sc-2044; Santa Cruz) in dPBS for 30 min at room temperature (RT) in a humidified chamber; primary antibody solution was 100 μL of monoclonal rabbit anti-human FAH IgG antibody (Cat. no. HPA-04137; Sigma) at 1:100 (Australia) or 1:500 (US) in 10% donkey serum incubated overnight at 4°C (US) or for 2 hr at RT (Australia); secondary antibody solution was 100 μL of donkey anti-rabbit Alexa Fluor 647 IgG antibody (Cat. no. {"type":"entrez-protein","attrs":{"text":"A31573","term_id":"87384","term_text":"pir||A31573"}} A31573 ; Invitrogen) at 1:500 along with Hoechst 33342 (Cat. no. H-3570; Molecular Probes) at 1:1,000 in PBS with tween (PBST) for 1 hr at RT in dark conditions (US), or 100 μL of donkey anti-rabbit Alexa Fluor 594 IgG antibody (Cat. no. {"type":"entrez-nucleotide","attrs":{"text":"A21207","term_id":"583479","term_text":"A21207"}} A21207 ; Invitrogen) at 1:500 in PBS for 1 hr at RT in dark conditions followed by DAPI at 80 ng/mL in PBS; and slides were mounted with 3 drops of ProLong Gold Antifade (Cat. no. {"type":"entrez-protein","attrs":{"text":"P36934","term_id":"549428","term_text":"P36934"}} P36934 ; Invitrogen) (US) or ProLong Diamond Antifade (Cat. no. {"type":"entrez-protein","attrs":{"text":"P36961","term_id":"547831","term_text":"P36961"}} P36961 ; Invitrogen) (Australia). .. Antibody validity controls included secondary-only staining and demonstration on positive control frozen Article Title: Recombinant adeno-associated virus capsids resistant to pre-existing human neutralizing antibodies Article Snippet: Modifications included: fixing slides in ice-cold methanol for 10 minutes rather than acetone at room temperature; blocking with 10% rather than 5% donkey serum (Santa Cruz, Cat#sc-2044) in dPBS for 30-min at RT in a humidified chamber; primary antibody solution was 100-μl of monoclonal rabbit anti-human FAH IgG antibody (Sigma, Cat#HPA-04137) at 1:100 (Australia) or 1:500 (U.S.) in 10% donkey serum incubated overnight at 4° C. (U.S.) or for 2-hrs at RT (Australia); secondary antibody solution was 100-μl of donkey anti-rabbit AlexaFluor 647 IgG antibody (Invitrogen, Cat#A31573) at 1:500 along with Hoechst 33342 (Molecular Probes, Cat#H-3570) at 1:1,000 in PBST for 1-hr at RT in dark conditions (U.S.), or 100-μl of donkey anti-rabbit AlexaFluor 594 IgG antibody (Invitrogen, Cat#A21207) at 1:500 in PBS for 1-hr at RT in dark conditions followed by DAPI at 80-ng/mL in PBS; slides were mounted with 3 drops of ProLong Gold Antifade (Invitrogen, Cat#P36934) (U.S.) or ProLong Diamond Antifade (Invitrogen, Cat#P36961) (Australia). .. Antibody validity controls included secondary-only staining, and demonstration on positive control frozen Positive Control:Article Title: Bioengineered AAV Capsids with Combined High Human Liver Transduction In Vivo and Unique Humoral Seroreactivity Article Snippet: Modifications included fixing slides in ice-cold methanol for 10 min rather than acetone at room temperature; blocking with 10% rather than 5% donkey serum (Cat. no. sc-2044; Santa Cruz) in dPBS for 30 min at room temperature (RT) in a humidified chamber; primary antibody solution was 100 μL of monoclonal rabbit anti-human FAH IgG antibody (Cat. no. HPA-04137; Sigma) at 1:100 (Australia) or 1:500 (US) in 10% donkey serum incubated overnight at 4°C (US) or for 2 hr at RT (Australia); secondary antibody solution was 100 μL of donkey anti-rabbit Alexa Fluor 647 IgG antibody (Cat. no. {"type":"entrez-protein","attrs":{"text":"A31573","term_id":"87384","term_text":"pir||A31573"}} A31573 ; Invitrogen) at 1:500 along with Hoechst 33342 (Cat. no. H-3570; Molecular Probes) at 1:1,000 in PBS with tween (PBST) for 1 hr at RT in dark conditions (US), or 100 μL of donkey anti-rabbit Alexa Fluor 594 IgG antibody (Cat. no. {"type":"entrez-nucleotide","attrs":{"text":"A21207","term_id":"583479","term_text":"A21207"}} A21207 ; Invitrogen) at 1:500 in PBS for 1 hr at RT in dark conditions followed by DAPI at 80 ng/mL in PBS; and slides were mounted with 3 drops of ProLong Gold Antifade (Cat. no. {"type":"entrez-protein","attrs":{"text":"P36934","term_id":"549428","term_text":"P36934"}} P36934 ; Invitrogen) (US) or ProLong Diamond Antifade (Cat. no. {"type":"entrez-protein","attrs":{"text":"P36961","term_id":"547831","term_text":"P36961"}} P36961 ; Invitrogen) (Australia). .. Antibody validity controls included secondary-only staining and demonstration on positive control frozen Article Title: Recombinant adeno-associated virus capsids resistant to pre-existing human neutralizing antibodies Article Snippet: Modifications included: fixing slides in ice-cold methanol for 10 minutes rather than acetone at room temperature; blocking with 10% rather than 5% donkey serum (Santa Cruz, Cat#sc-2044) in dPBS for 30-min at RT in a humidified chamber; primary antibody solution was 100-μl of monoclonal rabbit anti-human FAH IgG antibody (Sigma, Cat#HPA-04137) at 1:100 (Australia) or 1:500 (U.S.) in 10% donkey serum incubated overnight at 4° C. (U.S.) or for 2-hrs at RT (Australia); secondary antibody solution was 100-μl of donkey anti-rabbit AlexaFluor 647 IgG antibody (Invitrogen, Cat#A31573) at 1:500 along with Hoechst 33342 (Molecular Probes, Cat#H-3570) at 1:1,000 in PBST for 1-hr at RT in dark conditions (U.S.), or 100-μl of donkey anti-rabbit AlexaFluor 594 IgG antibody (Invitrogen, Cat#A21207) at 1:500 in PBS for 1-hr at RT in dark conditions followed by DAPI at 80-ng/mL in PBS; slides were mounted with 3 drops of ProLong Gold Antifade (Invitrogen, Cat#P36934) (U.S.) or ProLong Diamond Antifade (Invitrogen, Cat#P36961) (Australia). .. Antibody validity controls included secondary-only staining, and demonstration on positive control frozen Negative Control:Article Title: Bioengineered AAV Capsids with Combined High Human Liver Transduction In Vivo and Unique Humoral Seroreactivity Article Snippet: Modifications included fixing slides in ice-cold methanol for 10 min rather than acetone at room temperature; blocking with 10% rather than 5% donkey serum (Cat. no. sc-2044; Santa Cruz) in dPBS for 30 min at room temperature (RT) in a humidified chamber; primary antibody solution was 100 μL of monoclonal rabbit anti-human FAH IgG antibody (Cat. no. HPA-04137; Sigma) at 1:100 (Australia) or 1:500 (US) in 10% donkey serum incubated overnight at 4°C (US) or for 2 hr at RT (Australia); secondary antibody solution was 100 μL of donkey anti-rabbit Alexa Fluor 647 IgG antibody (Cat. no. {"type":"entrez-protein","attrs":{"text":"A31573","term_id":"87384","term_text":"pir||A31573"}} A31573 ; Invitrogen) at 1:500 along with Hoechst 33342 (Cat. no. H-3570; Molecular Probes) at 1:1,000 in PBS with tween (PBST) for 1 hr at RT in dark conditions (US), or 100 μL of donkey anti-rabbit Alexa Fluor 594 IgG antibody (Cat. no. {"type":"entrez-nucleotide","attrs":{"text":"A21207","term_id":"583479","term_text":"A21207"}} A21207 ; Invitrogen) at 1:500 in PBS for 1 hr at RT in dark conditions followed by DAPI at 80 ng/mL in PBS; and slides were mounted with 3 drops of ProLong Gold Antifade (Cat. no. {"type":"entrez-protein","attrs":{"text":"P36934","term_id":"549428","term_text":"P36934"}} P36934 ; Invitrogen) (US) or ProLong Diamond Antifade (Cat. no. {"type":"entrez-protein","attrs":{"text":"P36961","term_id":"547831","term_text":"P36961"}} P36961 ; Invitrogen) (Australia). .. Antibody validity controls included secondary-only staining and demonstration on positive control frozen Article Title: Recombinant adeno-associated virus capsids resistant to pre-existing human neutralizing antibodies Article Snippet: Modifications included: fixing slides in ice-cold methanol for 10 minutes rather than acetone at room temperature; blocking with 10% rather than 5% donkey serum (Santa Cruz, Cat#sc-2044) in dPBS for 30-min at RT in a humidified chamber; primary antibody solution was 100-μl of monoclonal rabbit anti-human FAH IgG antibody (Sigma, Cat#HPA-04137) at 1:100 (Australia) or 1:500 (U.S.) in 10% donkey serum incubated overnight at 4° C. (U.S.) or for 2-hrs at RT (Australia); secondary antibody solution was 100-μl of donkey anti-rabbit AlexaFluor 647 IgG antibody (Invitrogen, Cat#A31573) at 1:500 along with Hoechst 33342 (Molecular Probes, Cat#H-3570) at 1:1,000 in PBST for 1-hr at RT in dark conditions (U.S.), or 100-μl of donkey anti-rabbit AlexaFluor 594 IgG antibody (Invitrogen, Cat#A21207) at 1:500 in PBS for 1-hr at RT in dark conditions followed by DAPI at 80-ng/mL in PBS; slides were mounted with 3 drops of ProLong Gold Antifade (Invitrogen, Cat#P36934) (U.S.) or ProLong Diamond Antifade (Invitrogen, Cat#P36961) (Australia). .. Antibody validity controls included secondary-only staining, and demonstration on positive control frozen |